Popscle

Introduction

Popscle is a suite of population scale analysis tools for single-cell genomics data.

For more information, please check its | Docker hub: https://hub.docker.com/r/cumulusprod/popscle and its home page on Github.

Versions

0.1b

Commands

popscle

Module

You can load the modules by:

module load biocontainers
module load popscle

Example job

Warning

Using #!/bin/sh -l as shebang in the slurm job script will cause the failure of some biocontainer modules. Please use #!/bin/bash instead.

To run Popscle on our clusters:

#!/bin/bash
#SBATCH -A myallocation     # Allocation name
#SBATCH -t 1:00:00
#SBATCH -N 1
#SBATCH -n 1
#SBATCH --job-name=popscle
#SBATCH --mail-type=FAIL,BEGIN,END
#SBATCH --error=%x-%J-%u.err
#SBATCH --output=%x-%J-%u.out

module --force purge
ml biocontainers popscle

popscle dsc-pileup --sam data/$bam --vcf data/$ref_vcf --out data/$pileup