Dropest

Introduction

Dropest is a pipeline for initial analysis of droplet-based single-cell RNA-seq data.

For more information, please check its website: https://biocontainers.pro/tools/dropest and its home page on Github.

Versions

  • 0.8.6

Commands

  • dropest

  • droptag

  • dropReport.Rsc

  • R

  • Rscript

Module

You can load the modules by:

module load biocontainers
module load dropest

Example job

Warning

Using #!/bin/sh -l as shebang in the slurm job script will cause the failure of some biocontainer modules. Please use #!/bin/bash instead.

To run Dropest on our clusters:

#!/bin/bash
#SBATCH -A myallocation     # Allocation name
#SBATCH -t 1:00:00
#SBATCH -N 1
#SBATCH -n 1
#SBATCH --job-name=dropest
#SBATCH --mail-type=FAIL,BEGIN,END
#SBATCH --error=%x-%J-%u.err
#SBATCH --output=%x-%J-%u.out

module --force purge
ml biocontainers dropest

dropest -f -c 10x.xml  -C 1200 neurons_900_possorted_genome_bam.bam