FastQC

Introduction

FastQC aims to provide a simple way to do some quality control checks on raw sequence data coming from high throughput sequencing pipelines. It provides a modular set of analyses which you can use to give a quick impression of whether your data has any problems of which you should be aware before doing any further analysis.

For more information, please check its website: https://biocontainers.pro/tools/fastqc and its home page: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/.

Versions

  • 0.11.9

Commands

  • fastqc

Module

You can load the modules by:

module load biocontainers
module load fastqc

Example job

Warning

Using #!/bin/sh -l as shebang in the slurm job script will cause the failure of some biocontainer modules. Please use #!/bin/bash instead.

To run Fastqc on our clusters:

#!/bin/bash
#SBATCH -A myallocation     # Allocation name
#SBATCH -t 1:00:00
#SBATCH -N 1
#SBATCH -n 4
#SBATCH --job-name=fastqc
#SBATCH --mail-type=FAIL,BEGIN,END
#SBATCH --error=%x-%J-%u.err
#SBATCH --output=%x-%J-%u.out

module --force purge
ml biocontainers fastqc

fastqc -o fastqc_out -t 4 FASTQ1 FASTQ2