Last

Introduction

Last is used to find & align related regions of sequences.

For more information, please check its website: https://biocontainers.pro/tools/last and its home page on Gitlab.

Versions

  • 1268

  • 1356

  • 1411

  • 1418

Commands

  • last-dotplot

  • last-map-probs

  • last-merge-batches

  • last-pair-probs

  • last-postmask

  • last-split

  • last-split5

  • last-train

  • lastal

  • lastal5

  • lastdb

  • lastdb5

Module

You can load the modules by:

module load biocontainers
module load last

Example job

Warning

Using #!/bin/sh -l as shebang in the slurm job script will cause the failure of some biocontainer modules. Please use #!/bin/bash instead.

To run Last on our clusters:

#!/bin/bash
#SBATCH -A myallocation     # Allocation name
#SBATCH -t 1:00:00
#SBATCH -N 1
#SBATCH -n 1
#SBATCH --job-name=last
#SBATCH --mail-type=FAIL,BEGIN,END
#SBATCH --error=%x-%J-%u.err
#SBATCH --output=%x-%J-%u.out

module --force purge
ml biocontainers last

lastdb humdb humanMito.fa
lastal humdb fuguMito.fa > myalns.maf