Gmap

Introduction

Gmap is a genomic mapping and alignment program for mRNA and EST sequences.

For more information, please check its website: https://biocontainers.pro/tools/gmap and its home page: http://research-pub.gene.com/gmap/.

Versions

  • 2021.05.27

  • 2021.08.25

Commands

  • atoiindex

  • cmetindex

  • cpuid

  • dbsnp_iit

  • ensembl_genes

  • fa_coords

  • get-genome

  • gff3_genes

  • gff3_introns

  • gff3_splicesites

  • gmap

  • gmap.avx2

  • gmap_build

  • gmap_cat

  • gmapindex

  • gmapl

  • gmapl.avx2

  • gmapl.nosimd

  • gmap.nosimd

  • gmap_process

  • gsnap

  • gsnap.avx2

  • gsnapl

  • gsnapl.avx2

  • gsnapl.nosimd

  • gsnap.nosimd

  • gtf_genes

  • gtf_introns

  • gtf_splicesites

  • gtf_transcript_splicesites

  • gvf_iit

  • iit_dump

  • iit_get

  • iit_store

  • indexdb_cat

  • md_coords

  • psl_genes

  • psl_introns

  • psl_splicesites

  • sam_sort

  • snpindex

  • trindex

  • vcf_iit

Module

You can load the modules by:

module load biocontainers
module load gmap

Example job

Warning

Using #!/bin/sh -l as shebang in the slurm job script will cause the failure of some biocontainer modules. Please use #!/bin/bash instead.

To run Gmap on our clusters:

#!/bin/bash
#SBATCH -A myallocation     # Allocation name
#SBATCH -t 1:00:00
#SBATCH -N 1
#SBATCH -n 4
#SBATCH --job-name=gmap
#SBATCH --mail-type=FAIL,BEGIN,END
#SBATCH --error=%x-%J-%u.err
#SBATCH --output=%x-%J-%u.out

module --force purge
ml biocontainers gmap

gmap_build -d Cmm -D Cmm genome.fasta
gmap -d Cmm -t 4 -D ./Cmm  cdna.fasta > gmap_out.txt

gmap_build -d GRCh38 -D GRCh38 Homo_sapiens.GRCh38.dna.primary_assembly.fa
gsnap -d GRCh38 -D ./GRCh38 --nthreads=4  SRR16956239_1.fastq SRR16956239_2.fastq > gsnap_out.txt