Gmap
Introduction
Gmap
is a genomic mapping and alignment program for mRNA and EST sequences.
For more information, please check its website: https://biocontainers.pro/tools/gmap and its home page: http://research-pub.gene.com/gmap/.
Versions
2021.05.27
2021.08.25
Commands
atoiindex
cmetindex
cpuid
dbsnp_iit
ensembl_genes
fa_coords
get-genome
gff3_genes
gff3_introns
gff3_splicesites
gmap
gmap.avx2
gmap_build
gmap_cat
gmapindex
gmapl
gmapl.avx2
gmapl.nosimd
gmap.nosimd
gmap_process
gsnap
gsnap.avx2
gsnapl
gsnapl.avx2
gsnapl.nosimd
gsnap.nosimd
gtf_genes
gtf_introns
gtf_splicesites
gtf_transcript_splicesites
gvf_iit
iit_dump
iit_get
iit_store
indexdb_cat
md_coords
psl_genes
psl_introns
psl_splicesites
sam_sort
snpindex
trindex
vcf_iit
Module
You can load the modules by:
module load biocontainers
module load gmap
Example job
Warning
Using #!/bin/sh -l
as shebang in the slurm job script will cause the failure of some biocontainer modules. Please use #!/bin/bash
instead.
To run Gmap on our clusters:
#!/bin/bash
#SBATCH -A myallocation # Allocation name
#SBATCH -t 1:00:00
#SBATCH -N 1
#SBATCH -n 4
#SBATCH --job-name=gmap
#SBATCH --mail-type=FAIL,BEGIN,END
#SBATCH --error=%x-%J-%u.err
#SBATCH --output=%x-%J-%u.out
module --force purge
ml biocontainers gmap
gmap_build -d Cmm -D Cmm genome.fasta
gmap -d Cmm -t 4 -D ./Cmm cdna.fasta > gmap_out.txt
gmap_build -d GRCh38 -D GRCh38 Homo_sapiens.GRCh38.dna.primary_assembly.fa
gsnap -d GRCh38 -D ./GRCh38 --nthreads=4 SRR16956239_1.fastq SRR16956239_2.fastq > gsnap_out.txt