Isoquant

Introduction

IsoQuant is a tool for the genome-based analysis of long RNA reads, such as PacBio or Oxford Nanopores. IsoQuant allows to reconstruct and quantify transcript models with high precision and decent recall. If the reference annotation is given, IsoQuant also assigns reads to the annotated isoforms based on their intron and exon structure. IsoQuant further performs annotated gene, isoform, exon and intron quantification. If reads are grouped (e.g. according to cell type), counts are reported according to the provided grouping.

For more information, please check:

BioContainers: https://biocontainers.pro/tools/isoquant

Home page: https://github.com/ablab/IsoQuant

Versions

• 3.1.2

Commands

• isoquant.py

Module

You can load the modules by:

module load biocontainers
module load isoquant

Example job

Warning

Using #!/bin/sh -l as shebang in the slurm job script will cause the failure of some biocontainer modules. Please use #!/bin/bash instead.

To run isoquant on our clusters:

#!/bin/bash
#SBATCH -A myallocation     # Allocation name
#SBATCH -t 1:00:00
#SBATCH -N 1
#SBATCH -n 1
#SBATCH --job-name=isoquant
#SBATCH --mail-type=FAIL,BEGIN,END
#SBATCH --error=%x-%J-%u.err
#SBATCH --output=%x-%J-%u.out

module --force purge
ml biocontainers isoquant

isoquant.py --reference chr9.4M.fa.gz \
    --genedb chr9.4M.gtf.gz \
    --fastq  chr9.4M.ont.sim.fq.gz \
    --data_type nanopore -o test_ont